Genetic- and Fiber-Diet-Mediated Changes in Antibiotic Resistance Genes in Pig Colon Contents and Feces and Their Driving Factors

Comprehensive studies on the effects of genetics and fiber diets antibiotic resistance genes (ARGs) remain scarce. In this study, we analyzed profiles ARGs in colonic contents fecal samples Taoyuan, Duroc, Xiangcun pigs (n = 10) fed at different levels. Through macrogenomic analysis, identified a total 850 unique types classified them into 111 drug classes. The abundance partially drug-resistant was higher local pig breeds under large-scale farming model. were found to be widely distributed among variety bacteria, predominantly phyla Firmicutes, Proteobacteria, Bacteroidetes. Fiber reduce feces, mobile genetic elements (MGEs) short-chain fatty acids (SCFAs) are important drivers mediating effect ARGs. vitro fermentation experiments confirmed that butyric acid significantly reduced summary, results study enhanced our understanding distribution composition colon revealed diet can feces through its Butyric acid, providing reference data for environmental safety.